CBE6b-SpRY
(Plasmid #257826)

• Purpose: CBE6b Sp Cas9 SpRY IVT plasmid
• Depositing Lab: Gregory Newby
• Publication: Genome Editing in Primary Mammalian Cells via Electroporation of Editor RNA (unpublished)

Backbone

• Vector backbone: pT7
• Backbone size w/o insert (bp): 2464
• Total vector size (bp): 8857
• Modifications to backbone: Inactivated T7 promoter to prevent transcription from plasmid
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: CBE6b SpCas9 SpRY
• Alt name: CBE6b SpRY
• Insert Size (bp): 6393

Gene/Insert 2

• Gene/Insert name: CBE6b Sp Cas9 SpRY

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry