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pKG04155_Bxb1-GT_CAG-rtTA-T2A-mTagBFP2-bGH-(TRE3G-mScarlet-WPRE)
(Plasmid #252546)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252546 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    STRAIGHT-IN Bxb1 GT hEF1a donor
  • Backbone manufacturer
    Richard Davis and Katie Galloway labs
  • Backbone size w/o insert (bp) 4747
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rtTA-T2A-mTagBFP2
  • Alt name
    Tet-On 3G
  • Species
    Synthetic; Entacmaea quadricolor, Escherichia coli
  • Insert Size (bp)
    5338
  • Promoter CAG (human cytomegalovirus immediate early enhancer, chicken beta-actin promoter, and chimeric intron), TRE3G
  • Tag / Fusion Protein
    • SV40 NLSx3 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Richard Davis lab

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also expresses mScarlet. Please visit https://doi.org/10.1101/2025.01.19.633652 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKG04155_Bxb1-GT_CAG-rtTA-T2A-mTagBFP2-bGH-(TRE3G-mScarlet-WPRE) was a gift from Kate Galloway (Addgene plasmid # 252546 ; http://n2t.net/addgene:252546 ; RRID:Addgene_252546)

  • For your References section:

    Gene syntax defines supercoiling-mediated transcriptional feedback. Johnstone CP, Love KS, Kabaria SR, Jones R, Blanch-Asensio A, Ploessl DS, Peterman EL, Lee R, Yun J, Oakes CG, Mummery CL, Davis RP, DeKosky BJ, Zandstra PW, Galloway KE. bioRxiv [Preprint]. 2025 Jan 19:2025.01.19.633652. doi: 10.1101/2025.01.19.633652. 10.1101/2025.01.19.633652 PubMed 39868195
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