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pKG04062_pLentiX1-EF1alpha-GapR-3xFLAG-NLS-EGFP-bGH
(Plasmid #252547)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252547 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLentiX1
  • Backbone manufacturer
    Addgene #17297
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GapR-3xFLAG-NLS-EGFP
  • Alt name
    GapR
  • Alt name
    EGFP
  • Species
    Caulobacter vibrioides NA1000, Aequorea victoria
  • Insert Size (bp)
    2536
  • Promoter hEF1a (human elongation factor 1-alpha)
  • Tag / Fusion Protein
    • 3xFLAG-SV40 NLS (C terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.19.633652 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKG04062_pLentiX1-EF1alpha-GapR-3xFLAG-NLS-EGFP-bGH was a gift from Kate Galloway (Addgene plasmid # 252547 ; http://n2t.net/addgene:252547 ; RRID:Addgene_252547)

  • For your References section:

    Gene syntax defines supercoiling-mediated transcriptional feedback. Johnstone CP, Love KS, Kabaria SR, Jones R, Blanch-Asensio A, Ploessl DS, Peterman EL, Lee R, Yun J, Oakes CG, Mummery CL, Davis RP, DeKosky BJ, Zandstra PW, Galloway KE. bioRxiv [Preprint]. 2025 Jan 19:2025.01.19.633652. doi: 10.1101/2025.01.19.633652. 10.1101/2025.01.19.633652 PubMed 39868195
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