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pTW2279
(Plasmid #252580)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252580 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    T-DNA
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ymu1 with AtPDS3 gRNA 12 and AtCHLl1 gRNA 4

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.01.23.700382 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTW2279 was a gift from Steven Jacobsen (Addgene plasmid # 252580 ; http://n2t.net/addgene:252580 ; RRID:Addgene_252580)

  • For your References section:

    Efficient Transgene-Free Multiplexed Germline Editing via Viral Delivery of an Engineered TnpB. Weiss T, Kamalu M, Shi H, Wirnowski G, Ingelsson A, Amerasekera J, Vohra K, Trinidad MI, Li Z, Freitas E, Steinmetz N, Ambrose C, Chen K, Doudna JA, Jacobsen SE. Plant Biotechnol J. 2026 Mar 17. doi: 10.1111/pbi.70644. 10.1111/pbi.70644 PubMed 41841630
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