Skip to main content
Addgene
Open Menu

NSE-huCof K22Q-mRFP
(Plasmid #252618)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 252618 Standard format: Plasmid sent in bacteria as agar stab 1 $89 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pShuttle-NSE
  • Backbone size w/o insert (bp) 7981
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cofilin 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    CFL1 (a.k.a. CFL, HEL-S-15, cofilin)
  • Promoter Neuronal Specific Enolase (NSE)
  • Tag / Fusion Protein
    • mRFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Flynn et al. 2009 (doi: 10.1002/dneu.20734, PMID: 19513994); Forss-Petter et al. 1990 (doi: 10.1016/0896-6273(90)90308-3, PMID: 2116814)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For neuronal-specific expression of cofilin-mRFP, a cofilactin rod reporter.

Please note: This plasmid contains silent mutations at amino acids S23 and T25 in cofilin. These mutations are not known to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NSE-huCof K22Q-mRFP was a gift from James Bamburg (Addgene plasmid # 252618 ; http://n2t.net/addgene:252618 ; RRID:Addgene_252618)

  • For your References section:

    A genetically encoded reporter for real-time imaging of cofilin-actin rods in living neurons. Mi J, Shaw AE, Pak CW, Walsh KP, Minamide LS, Bernstein BW, Kuhn TB, Bamburg JR. PLoS One. 2013 Dec 31;8(12):e83609. doi: 10.1371/journal.pone.0083609. PubMed 24391794
Related items: