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p-cymR-cre[LVA]-loxPP-eyfp
(Plasmid #252620)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252620 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    p15A-Kan
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    cymRAM + PcymRC-cre[LVA] + PW4-loxP-TT-loxP-eyfp
  • Insert Size (bp)
    3425
  • Promoter PcymRC
  • Tag / Fusion Protein
    • cre[LVA] (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cymR^AM+ PcymRC + eyfp received from Christopher Voigt (Addgene plasmid #108513); cre recombinase received from Niels Geijsen (Addgene plasmid #62730); PW4 and loxP synthesized by Twist Bioscience, sequences are from Sheets et al. 2020 (doi: 10.1021/acssynbio.9b00395); TT terminators ECK120033736 and L3S2P11 were synthesized by Twist Bioscience, sequences are from Chen et al. 2013 (doi: 10.1038/nmeth.2515).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.10.02.616356 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-cymR-cre[LVA]-loxPP-eyfp was a gift from Andrea Giometto (Addgene plasmid # 252620 ; http://n2t.net/addgene:252620 ; RRID:Addgene_252620)

  • For your References section:

    Tunable Low-Rate Genomic Recombination with Cre-lox in Escherichia coli : A Versatile Tool for Environmental Biosensing and Synthetic Biology. Garabello E, Yoon H, Reid MC, Giometto A. bioRxiv [Preprint]. 2025 Sep 2:2024.10.02.616356. doi: 10.1101/2024.10.02.616356. 10.1101/2024.10.02.616356 PubMed 40949998
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