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p-ArsRBS2-cre[LVA]-loxPP-syfp2
(Plasmid #252621)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252621 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    p15A-Kan
  • Backbone manufacturer
    Christopher Voigt (Addgene plasmid #108517)
  • Modifications to backbone
    Added Ampicillin resistance gene
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ParsR-arsR-cre[LVA] + PrpsL-loxP-TT-loxP-syfp2
  • Insert Size (bp)
    2951
  • Promoter ParsR
  • Tag / Fusion Protein
    • cre[LVA] (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid backbone received from Christopher Voigt (Addgene plasmid #108517). ParsR-arsR received from Alexander Poulain; cre recombinase gene received from Niels Geijsen (Addgene plasmid #62730); TT terminators ECK120033736 and L3S2P11 were synthesized by Twist Bioscience, sequences are from Chen et al. 2013 (doi: 10.1038/nmeth.2515); PrpsL-syfp2 received from Johan Paulsson.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.10.02.616356 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-ArsRBS2-cre[LVA]-loxPP-syfp2 was a gift from Andrea Giometto (Addgene plasmid # 252621 ; http://n2t.net/addgene:252621 ; RRID:Addgene_252621)

  • For your References section:

    Tunable Low-Rate Genomic Recombination with Cre-lox in Escherichia coli : A Versatile Tool for Environmental Biosensing and Synthetic Biology. Garabello E, Yoon H, Reid MC, Giometto A. bioRxiv [Preprint]. 2025 Sep 2:2024.10.02.616356. doi: 10.1101/2024.10.02.616356. 10.1101/2024.10.02.616356 PubMed 40949998
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