pSLQ12774-PHR-EF1a-leucine zipper-GFP11-NES-2A-CD19 (no ICD)-P2A-puroR-2A-BFP-NLS
(Plasmid
#252642)
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PurposeHuman expression vector containing EF1a promoter, GFP11 along with a leucine zipper, CD19 (no intracellular domain), nuclear BFP, and puromycin resistance gene
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252642 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonePHR
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP11-leucine-zipper, CD19 (no ICD), PuroR, BFP-NLS
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SpeciesH. sapiens (human)
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.03.12.642522 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLQ12774-PHR-EF1a-leucine zipper-GFP11-NES-2A-CD19 (no ICD)-P2A-puroR-2A-BFP-NLS was a gift from Stanley Qi (Addgene plasmid # 252642 ; http://n2t.net/addgene:252642 ; RRID:Addgene_252642) -
For your References section:
Programmable macromolecule delivery via engineered trogocytosis. Chen X, Situ Y, Yang Y, Fu ML, Lyu L, Qi LS. bioRxiv [Preprint]. 2025 Mar 14:2025.03.12.642522. doi: 10.1101/2025.03.12.642522. 10.1101/2025.03.12.642522 PubMed 40161588