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pOPINTTGneoFc
(Plasmid #252742)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252742 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
  • Backbone size w/o insert (bp) 8002
  • Total vector size (bp) 8706
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IgG1 Fc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    732
  • Entrez Gene
    IGHG1
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TCC ACA GGT GTC CAC TCC
  • 3′ sequencing primer TCC TTT ATT AGC CAG AGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINTTGneoFc was a gift from Ray Owens (Addgene plasmid # 252742 ; http://n2t.net/addgene:252742 ; RRID:Addgene_252742)

  • For your References section:

    Overview of a High-Throughput Pipeline for Streamlining the Production of Recombinant Proteins. Nettleship JE, Rada H, Owens RJ. Methods Mol Biol. 2019;2025:33-49. doi: 10.1007/978-1-4939-9624-7_2. 10.1007/978-1-4939-9624-7_2 PubMed 31267447
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