pcdna3_HA-KLC1(TPR6)
(Plasmid
#252745)
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PurposeExpression of the TPR repeats of KLC1 which can act as a dominant negative inhibitor of kinesin-1 function.
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252745 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepcdna3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKLC1
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SpeciesR. norvegicus (rat)
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MutationTPR region only
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Entrez GeneKlc1 (a.k.a. Kns2)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcdna3_HA-KLC1(TPR6) was a gift from Tom Rapoport & Kristen Verhey (Addgene plasmid # 252745 ; http://n2t.net/addgene:252745 ; RRID:Addgene_252745) -
For your References section:
Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules. Verhey KJ, Meyer D, Deehan R, Blenis J, Schnapp BJ, Rapoport TA, Margolis B. J Cell Biol. 2001 Mar 5;152(5):959-70. doi: 10.1083/jcb.152.5.959. 10.1083/jcb.152.5.959 PubMed 11238452