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pN1_NUP62-EGFP3
(Plasmid #252748)

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Item Catalog # Description Quantity Price (USD)
Plasmid 252748 Standard format: Plasmid sent in bacteria as agar stab 1 $89 *
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Backbone

  • Vector backbone
    pN1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NUP62
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Nup62 (a.k.a. D7Ertd649e, Nupc1, p62)
  • Tag / Fusion Protein
    • E3xGFP (C terminal on insert)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

plasmid based on PMID: 15502822

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pN1_NUP62-EGFP3 was a gift from Kristen Verhey (Addgene plasmid # 252748 ; http://n2t.net/addgene:252748 ; RRID:Addgene_252748)

  • For your References section:

    An assay for clogging the ciliary pore complex distinguishes mechanisms of cytosolic and membrane protein entry. Takao D, Dishinger JF, Kee HL, Pinskey JM, Allen BL, Verhey KJ. Curr Biol. 2014 Oct 6;24(19):2288-94. doi: 10.1016/j.cub.2014.08.012. Epub 2014 Sep 25. 10.1016/j.cub.2014.08.012 PubMed 25264252
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