pN1_Rev-GR(m10)-EGFP
(Plasmid
#252751)
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PurposeControl for studying nuclear export. Expression of a fusion protein containing full-length Rev, the hormone-responsive element of the rat Gr, and GFP. The m10 mutation abolishes nuclear export.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252751 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * | |
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Backbone
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Vector backbonepN1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHIV-1 Rev and Glucocorticoid Receptor (GR)
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SpeciesR. norvegicus (rat)
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Mutationm10 is mutation of nuclear export sequence
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
plasmid originally from PMID: 9724751
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pN1_Rev-GR(m10)-EGFP was a gift from Kristen Verhey (Addgene plasmid # 252751 ; http://n2t.net/addgene:252751 ; RRID:Addgene_252751) -
For your References section:
An assay for clogging the ciliary pore complex distinguishes mechanisms of cytosolic and membrane protein entry. Takao D, Dishinger JF, Kee HL, Pinskey JM, Allen BL, Verhey KJ. Curr Biol. 2014 Oct 6;24(19):2288-94. doi: 10.1016/j.cub.2014.08.012. Epub 2014 Sep 25. 10.1016/j.cub.2014.08.012 PubMed 25264252
