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pHIE822 pCMV Bglobin intron VSVGmut K47Q R354A in pMD2
(Plasmid #252774)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 252774 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMD2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VSVG K47Q R354A
  • Species
    Synthetic
  • Mutation
    K47Q, R354A
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Leonard Lab plasmid reference number: L4435 . Please visit https://doi.org/10.1101/2025.07.02.662894 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIE822 pCMV Bglobin intron VSVGmut K47Q R354A in pMD2 was a gift from Joshua Leonard (Addgene plasmid # 252774 ; http://n2t.net/addgene:252774 ; RRID:Addgene_252774)

  • For your References section:

    Distinguishing Pseudotransduction and True Transduction Enables Characterization and Bioengineering of Extracellular Vesicle-Adeno-Associated Virus Vectors. Boucher JD, Stranford DM, Edelstein HI, Tullman-Ercek D, Kamat NP, Leonard JN. J Extracell Vesicles. 2026 Apr;15(4):e70258. doi: 10.1002/jev2.70258. 10.1002/jev2.70258 PubMed 41942279
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