AAVS1 gRNAs-Cas9D10A-mRuby
(Plasmid
#252799)
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PurposePlasmid for paired targeting of AAVS1 locus via Cas9(D10A)nickase
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252799 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepX446
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9(D10A)
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gRNA/shRNA sequenceAAVS1
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- mRuby
Resource Information
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A portion of this plasmid was derived from a plasmid made byFrom Jonathon Pine's Lab at The Institute of Cancer Research
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAVS1 gRNAs-Cas9D10A-mRuby was a gift from Norman Davey (Addgene plasmid # 252799 ; http://n2t.net/addgene:252799 ; RRID:Addgene_252799)