AAVS1 locus donor plasmid
(Plasmid
#252870)
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PurposePlasmid encoding FRT recombination site and TetOn3G transactivator flanked by AAVS1 homology arms
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252870 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepDrive
- Total vector size (bp) 7932
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTetOn3G transactivator and FRT recombination site
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SpeciesH. sapiens (human)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Homology arms sequence kindly provided by Konstantinos Anastassiadis' Lab
TetOn3G transactivator sequence comes from TAKARA TetOn3G system
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAVS1 locus donor plasmid was a gift from Norman Davey (Addgene plasmid # 252870 ; http://n2t.net/addgene:252870 ; RRID:Addgene_252870)