5-HT2C_IGV
(Plasmid
#252891)
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PurposeMammalian expression of RNA edited version of human 5-HT2C
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252891 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5-HT2C
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SpeciesH. sapiens (human)
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Insert Size (bp)1404
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MutationIGV isoform
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Entrez GeneHTR2C (a.k.a. 5-HT1C, 5-HT2C, 5-HTR2C, 5HTR2C, HTR1C)
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Tag
/ Fusion Protein
- HA tag, inserted after signal peptide (N terminal on insert)
Cloning Information
- Cloning method Other
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
5-HT2C_IGV was a gift from Cesare Orlandi (Addgene plasmid # 252891 ; http://n2t.net/addgene:252891 ; RRID:Addgene_252891) -
For your References section:
Serotonin 5-HT(2C) Receptor Signaling Analysis Reveals Psychedelic Biased Agonism. Bonniwell EM, Alabdali R, Hennessey JJ, McKee JL, Cavalco NG, Lammers JC, Moore EJ, Franchini L, Orlandi C, McCorvy JD. ACS Chem Neurosci. 2025 Oct 1;16(19):3899-3914. doi: 10.1021/acschemneuro.5c00647. Epub 2025 Sep 13. 10.1021/acschemneuro.5c00647 PubMed 40944639