pTipi2.2d
(Plasmid
#252902)
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PurposeGolden gate destination vector, allows for expression of recombinant proteins, no tags
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252902 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * | |
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Backbone
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Vector backbonepTipi2.2a
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Backbone manufacturerInstitute for Protein Innovation (IPI)
- Backbone size w/o insert (bp) 4914
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameConstitutive GFP
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SpeciesE. coli
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Insert Size (bp)1042
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer WPRE-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PaqCI cutsites, 5' OH = CTCC, 3' OH = AGGA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTipi2.2d was a gift from Institute for Protein Innovation (IPI) (Addgene plasmid # 252902 ; http://n2t.net/addgene:252902 ; RRID:Addgene_252902) -
For your References section:
Evaluating codon optimization strategies for mammalian glycoprotein production with an open-source expression vector. Yang C, Soni R, Visconti SE, Abdollahi M, Belay F, Ghosh A, Duvall SW, Walton CJW, Meijers R, Zhu H. bioRxiv, March 20, 2026 10.64898/2026.03.18.712111