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pMB.PRC1.4_RING1B_MBP-PCGF4
(Plasmid #252957)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252957 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMB.BIG1a
  • Total vector size (bp) 9900
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Spectinomycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    BMI1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2150
  • Entrez Gene
    BMI1 (a.k.a. FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1)
  • Promoter polH
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    RNF2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1210
  • Entrez Gene
    RNF2 (a.k.a. BAP-1, BAP1, DING, HIPI3, LUSYAM, RING1B, RING2)
  • Promoter polH
  • Tag / Fusion Protein
    • His-Dual-Strep (N terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMB.PRC1.4_RING1B_MBP-PCGF4 was a gift from Chen Davidovich (Addgene plasmid # 252957 ; http://n2t.net/addgene:252957 ; RRID:Addgene_252957)

  • For your References section:

    DOT1L provides transcriptional memory through PRC1.1 antagonism. Neville D, Ferguson DT, Heikamp EB, Lai Z, Magor GW, Lam C, Dobbs OG, Levina V, Knezevic K, The JJ, Alex S, Suits SC, Rumler B, Uckelmann M, Talarmain L, Lam EYN, Perkins AC, Armstrong SA, Bell CC, Davidovich C, Gilan O. Nat Cell Biol. 2026 Feb 3. doi: 10.1038/s41556-025-01859-8. 10.1038/s41556-025-01859-8 PubMed 41634341
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