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pFY20
(Plasmid #253057)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253057 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    low copy number, episomal cloning vector
  • Selectable markers
    ura4 (fission yeast homolog of budding yeast ura3)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

this shuttle vector is for use in fission yeast cloning and library production

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFY20 was a gift from Mari Davidson & Wayne Wahls (Addgene plasmid # 253057 ; http://n2t.net/addgene:253057 ; RRID:Addgene_253057)

  • For your References section:

    Low-copy episomal vector pFY20 and high-saturation coverage genomic libraries for the fission yeast Schizosaccharomyces pombe. Wahls WP, Davidson MK. Yeast. 2008 Sep;25(9):643-50. doi: 10.1002/yea.1605. 10.1002/yea.1605 PubMed 18613214
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