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pFlagTurboNTD(N3)-MYPT1
(Plasmid #253072)

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Item Catalog # Description Quantity Price (USD)
Plasmid 253072 Standard format: Plasmid sent in bacteria as agar stab 1 $89 *
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Backbone

  • Vector backbone
    pFlagTurboCTD(N3)
  • Backbone manufacturer
    Laura Trinkle-Mulcahy
  • Backbone size w/o insert (bp) 4575
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ASPP2
  • Alt name
    PPP1R12A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3090
  • Entrez Gene
    PPP1R12A (a.k.a. GUBS, M130, MBS, MYPT1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FlagTurboNTD (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GTACATCAATGGGCGTGGATAG
  • 3′ sequencing primer GCTGTTCGCCACTATG
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFlagTurboNTD(N3)-MYPT1 was a gift from Laura Trinkle-Mulcahy (Addgene plasmid # 253072 ; http://n2t.net/addgene:253072 ; RRID:Addgene_253072)
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