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CRISPRi sgPTPN11-1
(Plasmid #253090)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253090 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    CRISPRi
  • Vector type
    CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTPN11
  • gRNA/shRNA sequence
    GGAGGAACATGACATCGCGG
  • Species
    H. sapiens (human)
  • Entrez Gene
    PTPN11 (a.k.a. BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRISPRi sgPTPN11-1 was a gift from Harish Vasudevan (Addgene plasmid # 253090 ; http://n2t.net/addgene:253090 ; RRID:Addgene_253090)

  • For your References section:

    A transcriptomic, proteomic, and functional genetic atlas dissects neurofibromin function in the peripheral nervous system. Vasudevan HN, Arang N, Sacconi Nunez M, Kennedy P, Payne E, Mohabeer S, Chien J, Wright A, Sale MJ, Krogan NJ, Forget A, McCormick F. Proc Natl Acad Sci U S A. 2025 Jul 8;122(27):e2506823122. doi: 10.1073/pnas.2506823122. Epub 2025 Jun 30. 10.1073/pnas.2506823122 PubMed 40587782
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