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pSynITR_DD
(Plasmid #253227)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253227 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC18
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Enhanced Green Fluorescent Protein
  • Alt name
    eGFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    720

Gene/Insert 2

  • Gene/Insert name
    SynITR
  • Species
    synthetic
  • Insert Size (bp)
    167

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer GCAGATTGTACTGAGAGTGC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSynITR_DD was a gift from Matthew Hirsch (Addgene plasmid # 253227 ; http://n2t.net/addgene:253227 ; RRID:Addgene_253227)

  • For your References section:

    AAV vector transduction restriction and attenuated toxicity in hESCs via a rationally designed inverted terminal repeat. Song L, Hasegawa T, Brown NJ, Bower JJ, Samulski RJ, Hirsch ML. Nucleic Acids Res. 2025 Jan 24;53(3):gkaf013. doi: 10.1093/nar/gkaf013. 10.1093/nar/gkaf013 PubMed 39868534
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