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pLV hU6-gRNA hUbC-dSpCas9-2xVP64-2A-PuroR
(Plasmid #253229)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253229 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    FUGW
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Humanized VP64-dSpCas9-VP64-2A-PuroR

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.11.681828 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV hU6-gRNA hUbC-dSpCas9-2xVP64-2A-PuroR was a gift from Charles Gersbach (Addgene plasmid # 253229 ; http://n2t.net/addgene:253229 ; RRID:Addgene_253229)

  • For your References section:

    Comprehensive profiling of transcription factors for reprogramming human astrocytes to neuronal cells through endogenous CRISPR-based gene activation. Reisman SJ, Halabi D, Miller SE, Song L, Geraghty S, Sangvai N, Rice G, Safi A, Crawford GE, Gersbach CA. bioRxiv 2025.10.11.681828 10.1101/2025.10.11.681828
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