PGK-LAMP1-mEos3.2
(Plasmid
#253233)
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PurposeOverexpress LAMP1 fused to the photoconvertible fluorescent protein mEos3.2.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253233 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneunknown
- Backbone size w/o insert (bp) 9263
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Vector typeLentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLAMP1
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SpeciesH. sapiens (human)
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Insert Size (bp)1251
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Entrez GeneLAMP1 (a.k.a. CD107a, LAMPA, LGP120)
- Promoter PGK
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Tag
/ Fusion Protein
- mEos3.2 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PGK-LAMP1-mEos3.2 was a gift from May Malicdan (Addgene plasmid # 253233 ; http://n2t.net/addgene:253233 ; RRID:Addgene_253233)