May Malicdan Lab: Malicdan Lab Plasmids 2026
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253233 | PGK-LAMP1-mEos3.2 | Overexpress LAMP1 fused to the photoconvertible fluorescent protein mEos3.2. |
| 253234 | PGK-SYNGR1-mNeonGreen | Overexpress Synaptogyrin 1 fused to mNeonGreen fluorescent tag to track neuronal presynaptic vesicles. |
| 253235 | PGK-NbALFA-mScarlet | Overexpress mScarlet-tagged NbALFA (ALFA-tag-binding nanobody) to enable live-cell imaging of ALFA-tagged proteins. |
| 253236 | EF1a-NbALFA-mScarlet | Overexpress mScarlet-tagged NbALFA (ALFA-tag-binding nanobody) to enable live-cell imaging of ALFA-tagged proteins. |
| 253237 | EF1a-NbALFA-HaloTag | Overexpress HaloTag-tagged NbALFA (ALFA-tag-binding nanobody) to enable live-cell imaging of ALFA-tagged proteins. |
| 253238 | PGK-NbALFA-HaloTag | Overexpress HaloTag-tagged NbALFA (ALFA-tag-binding nanobody) to enable live-cell imaging of ALFA-tagged proteins. |
| 253240 | PB-3xFLAG | Empty plasmid or Plasmid control. |
| 253241 | PGK-LifeAct-mScarlet | Overexpress LifeAct fused with mScarlet Fluorescent marker to visualize F-actin. |
| 253242 | PGK-LAMP1-APEX2 | Express LAMP1 fused to APEX2 peroxidase to map the spatial organization of the lysosomal membrane and its associated proteins. |
| 253244 | PGK-LAMP1-AlfaTag-APEX2 | Express LAMP1 fused to APEX2 peroxidase to map the spatial organization of the lysosomal membrane and its associated proteins. LAMP1 is also fused to AlfaTag for IF or WB detection. |
| 253245 | PGK-mNeonGreen-RAB27A | Overexpress RAB27A with an N-terminal mScarlet fluorescent tag. |
| 253246 | PGK-mScarlet-RAB27A | Overexpress RAB27A with an N-terminal mScarlet fluorescent tag. |
| 253249 | PB-LAMP1-3xFLAG-APEX2 | Express LAMP1 fused to APEX2 peroxidase to map the spatial organization of the lysosomal membrane and its associated proteins. LAMP1 is also fused to 3xFlag for IF or WB detection. |
| 253254 | PB-3xFLAG-2xStrepTag-LYST | Overexpress LYST with an N-terminal 3xFLAG-2xStrepTag. |