PB-LAMP1-3xFLAG-APEX2
(Plasmid
#253249)
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PurposeExpress LAMP1 fused to APEX2 peroxidase to map the spatial organization of the lysosomal membrane and its associated proteins. LAMP1 is also fused to 3xFlag for IF or WB detection.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonePB-3xFLAG-2xStrepTag (Addgene # 253239)
- Backbone size w/o insert (bp) 12659
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Vector typeMammalian Expression ; PiggyBac Transposon
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Selectable markersHygromycin ; mycNLS-mNeonGreen
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLAMP1
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SpeciesH. sapiens (human)
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Insert Size (bp)1251
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Entrez GeneLAMP1 (a.k.a. CD107a, LAMPA, LGP120)
- Promoter CAG
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Tag
/ Fusion Protein
- 3xFALG-APEX2 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-LAMP1-3xFLAG-APEX2 was a gift from May Malicdan (Addgene plasmid # 253249 ; http://n2t.net/addgene:253249 ; RRID:Addgene_253249)
