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PB-LAMP1-3xFLAG-APEX2
(Plasmid #253249)

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  • Purpose
    Express LAMP1 fused to APEX2 peroxidase to map the spatial organization of the lysosomal membrane and its associated proteins. LAMP1 is also fused to 3xFlag for IF or WB detection.
  • Depositing Lab
    May Malicdan
  • Depositing Organization
    National Human Genome Research Institute (NHGRI)
    Located in United States of America
  • Publication
    Malicdan Lab Plasmids 2026 (unpublished) ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253249 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    PB-3xFLAG-2xStrepTag (Addgene # 253239)
  • Backbone size w/o insert (bp) 12659
  • Vector type
    Mammalian Expression ; PiggyBac Transposon
  • Selectable markers
    Hygromycin ; mycNLS-mNeonGreen

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LAMP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1251
  • Entrez Gene
    LAMP1 (a.k.a. CD107a, LAMPA, LGP120)
  • Promoter CAG
  • Tag / Fusion Protein
    • 3xFALG-APEX2 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-LAMP1-3xFLAG-APEX2 was a gift from May Malicdan (Addgene plasmid # 253249 ; http://n2t.net/addgene:253249 ; RRID:Addgene_253249)
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