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pA_higher-CheY-pMag-mNeonGreen
(Plasmid #253260)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253260 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEB2
  • Backbone manufacturer
    J. H. Davis, A. J. Rubin, R. T. Sauer, Design, construction and characterization of a set of insulated bacterial promoters.
  • Backbone size w/o insert (bp) 3499
  • Total vector size (bp) 4593
  • Modifications to backbone
    E. Balleza, J. M. Kim, P. Cluzel, Systematic characterization of maturation time of fluorescent proteins in living cells. Nat Methods 15, 47-51 (2018). A.M. Vrabioiu, B.G. Hosu, A.D.T. Samuel, The dynamic response of the bacterial flagellar motor to its direct intracellular input. PNAS in press.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CheY_pMag_mNeonGreen
  • Species
    Synthetic; E. coli
  • GenBank ID
    946393 KC295282.1
  • Tag / Fusion Protein
    • pMag and mNeonGreen

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.28.684865 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pA_higher-CheY-pMag-mNeonGreen was a gift from Aravinthan DT Samuel (Addgene plasmid # 253260 ; http://n2t.net/addgene:253260 ; RRID:Addgene_253260)

  • For your References section:

    The dynamic response of the bacterial flagellar motor to its direct intracellular input signal. Vrabioiu AM, Hosu BG, Samuel ADT. Proc Natl Acad Sci U S A. 2026 Mar 10;123(10):e2516278123. doi: 10.1073/pnas.2516278123. Epub 2026 Mar 3. 10.1073/pnas.2516278123 PubMed 41774795
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