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pNCMV 16E6no* D64N
(Plasmid #253267)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253267 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    cmv bam neo
  • Backbone size w/o insert (bp) 6589
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPV 16E6
  • Alt name
    HpV16gp1
  • Alt name
    E6
  • Species
    HPV
  • Insert Size (bp)
    450
  • Mutation
    D64N
  • Entrez Gene
    E6 (a.k.a. HpV16gp1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag-HA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNCMV 16E6no* D64N was a gift from Karl Munger (Addgene plasmid # 253267 ; http://n2t.net/addgene:253267 ; RRID:Addgene_253267)

  • For your References section:

    Characterization of patient-derived HPV16 E6 and E7 variant alleles. Grace M, Beeravolu H, Alhamshari A, Skelin J, Kiessling S-Y, Bohm A, White EA, Munger K. J Virol. 2026 Mar 30:e0023626. doi: 10.1128/jvi.00236-26. 10.1128/jvi.00236-26 PubMed 41910269
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