CMV-C 16E7 H51N
(Plasmid
#253279)
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PurposeMammalian expression of HPV 16E7 with point mutation H51N
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonecmv bam neo
- Backbone size w/o insert (bp) 6589
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV 16E7
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Alt nameHpV16gp2
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Alt nameE7
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SpeciesHPV
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Insert Size (bp)300
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MutationH51N
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Entrez GeneE7 (a.k.a. HpV16gp2)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag-HA (C terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer B-globin F
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-C 16E7 H51N was a gift from Karl Munger (Addgene plasmid # 253279 ; http://n2t.net/addgene:253279 ; RRID:Addgene_253279) -
For your References section:
Characterization of patient-derived HPV16 E6 and E7 variant alleles. Grace M, Beeravolu H, Alhamshari A, Skelin J, Kiessling S-Y, Bohm A, White EA, Munger K. J Virol. 2026 Mar 30:e0023626. doi: 10.1128/jvi.00236-26. 10.1128/jvi.00236-26 PubMed 41910269