pT-Sticky
(Plasmid
#253286)
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PurposepTrc99A with FlgE_RRR ( 'sticky' hook) insert
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253286 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepTrc99A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFlgE_RRR
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Alt name'sticky' hook
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SpeciesE. coli
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GenBank IDNP_415594
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.10.28.684865 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT-Sticky was a gift from Aravinthan DT Samuel (Addgene plasmid # 253286 ; http://n2t.net/addgene:253286 ; RRID:Addgene_253286) -
For your References section:
The dynamic response of the bacterial flagellar motor to its direct intracellular input signal. Vrabioiu AM, Hosu BG, Samuel ADT. Proc Natl Acad Sci U S A. 2026 Mar 10;123(10):e2516278123. doi: 10.1073/pnas.2516278123. Epub 2026 Mar 3. 10.1073/pnas.2516278123 PubMed 41774795