pN1_FKBP-HsKIF1C(349-1103)-mCh
(Plasmid
#253321)
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Purposemammalian expression of the stalk+tail domains (aa 349-1103) of HsKIF1C tagged with FKBP and mCherry. The FKBP tag enables rapamycin-induced attachment to FRB-tagged proteins.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253321 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * | |
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Backbone
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Vector backbonepmCherry-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHsKIF1C(349-1103)
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SpeciesH. sapiens (human)
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Entrez GeneKIF1C (a.k.a. LTXS1, SATX2, SAX2, SPAX2, SPG58)
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Tags
/ Fusion Proteins
- FKBP (N terminal on insert)
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pN1_FKBP-HsKIF1C(349-1103)-mCh was a gift from Kristen Verhey (Addgene plasmid # 253321 ; http://n2t.net/addgene:253321 ; RRID:Addgene_253321) -
For your References section:
Multi-kinesin clusters impart mechanical stress that reveals mechanisms of microtubule breakage in cells. Geng Q, Bonilla A, Sandwith SN, Verhey KJ. J Cell Biol. 2025 Oct 6;224(10):e202501070. doi: 10.1083/jcb.202501070. Epub 2025 Aug 11. 10.1083/jcb.202501070 PubMed 40788221