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pN1_FKBP-mCh-FTH1
(Plasmid #253323)

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Item Catalog # Description Quantity Price (USD)
Plasmid 253323 Standard format: Plasmid sent in bacteria as agar stab 1 $89 *
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Backbone

  • Vector backbone
    pmCherry-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HsFTH1
  • Species
    H. sapiens (human)
  • Entrez Gene
    FTH1 (a.k.a. FHC, FTH, FTHL6, HFE5, NBIA9, PIG15, PLIF)
  • Tag / Fusion Protein
    • FKBP, mCherry (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pN1_FKBP-mCh-FTH1 was a gift from Kristen Verhey (Addgene plasmid # 253323 ; http://n2t.net/addgene:253323 ; RRID:Addgene_253323)

  • For your References section:

    Multi-kinesin clusters impart mechanical stress that reveals mechanisms of microtubule breakage in cells. Geng Q, Bonilla A, Sandwith SN, Verhey KJ. J Cell Biol. 2025 Oct 6;224(10):e202501070. doi: 10.1083/jcb.202501070. Epub 2025 Aug 11. 10.1083/jcb.202501070 PubMed 40788221
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