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V12_ftnA-ftnA
(Plasmid #253338)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253338 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pJ414
  • Backbone size w/o insert (bp) 4042
  • Total vector size (bp) 6636
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    V12-ftnA-ftnA
  • Species
    E. coli
  • Entrez Gene
    ftnA (a.k.a. b1905, ECK1904, ftn, gen-165, rsgA)
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CACGAAAACCTGTATTTTCAAggctctgatcagttgtataagcagatggtgca
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.01.16.700029 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V12_ftnA-ftnA was a gift from Qiangjun Zhou (Addgene plasmid # 253338 ; http://n2t.net/addgene:253338 ; RRID:Addgene_253338)

  • For your References section:

    Fully genetically encoded low-molecular-weight protein tags with defined shapes for direct molecular identification by cryo-electron tomography. Luo F, Sun R, Chalkley O, Li P, Zhou Q. bioRxiv [Preprint]. 2026 Jan 20:2026.01.16.700029. doi: 10.64898/2026.01.16.700029. 10.64898/2026.01.16.700029 PubMed 41648103
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