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3HA-ERHBH-HoxA7
(Plasmid #253603)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253603 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    MSCV-neo
  • Total vector size (bp) 8271
  • Vector type
    Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hoxa7
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1750
  • GenBank ID
    NM_001419269.1
  • Entrez Gene
    Hoxa7 (a.k.a. Hox-1.1, M6)
  • Promoter MSCV
  • Tag / Fusion Protein
    • 3xHA-ERHBD (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer MSCV-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3HA-ERHBH-HoxA7 was a gift from Hans Haecker (Addgene plasmid # 253603 ; http://n2t.net/addgene:253603 ; RRID:Addgene_253603)

  • For your References section:

    Murine hematopoietic progenitor cell lines with erythroid and megakaryocyte potential. Wu R, Salehi F, Redecke V, Ma Z, Marchetti M, Finkelstein D, Xu P, Cheng Y, Queisser KA, Petrey AC, Field CO, Ahn HS, Poncz M, Weiss MJ, Hacker H. Nat Commun. 2025 Aug 7;16(1):7283. doi: 10.1038/s41467-025-62668-z. 10.1038/s41467-025-62668-z PubMed 40775216
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