CMV-C
(Plasmid
#253674)
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Purpose(Empty Backbone) For cloning, adds C-terminal Flag/HA tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253674 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepCMV-Neo-Bam
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Backbone manufacturerBert Vogelstein (Addgene # 16440)
- Backbone size (bp) 6500
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Modifications to backboneAdded Flag/HA linker at BamHI site
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV bam neo 5'
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-C was a gift from Karl Munger (Addgene plasmid # 253674 ; http://n2t.net/addgene:253674 ; RRID:Addgene_253674) -
For your References section:
The HPV101 E7 protein shares host cellular targets and biological activities with high-risk HPV16 E7. Gelbard MK, Grace M, von Schoeler-Ames A, Gnanou I, Munger K. Tumour Virus Res. 2025 Jun;19:200300. doi: 10.1016/j.tvr.2024.200300. Epub 2024 Dec 5. 10.1016/j.tvr.2024.200300 PubMed 39643241
Map uploaded by the depositor.
