pLenti-CMV-Blast-DEST C-HPV101 E7
(Plasmid
#253676)
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PurposeExpresses C-Flag/HA HPV101 E7 in lentiviral background
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253676 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLenti-CMV-Blast-Dest
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Backbone manufacturerEric Campeau, Paul Kaufman (Addgene #17451)
- Backbone size w/o insert (bp) 9331
- Total vector size (bp) 8094
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV101 E7
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Alt nameHPV101_gp1
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SpeciesHPV101
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Insert Size (bp)296
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GenBank IDNC_008189.1
- Promoter CMV
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Tag
/ Fusion Protein
- Flag/HA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV F
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-CMV-Blast-DEST C-HPV101 E7 was a gift from Karl Munger (Addgene plasmid # 253676 ; http://n2t.net/addgene:253676 ; RRID:Addgene_253676) -
For your References section:
The HPV101 E7 protein shares host cellular targets and biological activities with high-risk HPV16 E7. Gelbard MK, Grace M, von Schoeler-Ames A, Gnanou I, Munger K. Tumour Virus Res. 2025 Jun;19:200300. doi: 10.1016/j.tvr.2024.200300. Epub 2024 Dec 5. 10.1016/j.tvr.2024.200300 PubMed 39643241