|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25371||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBabe IRES puro
- Backbone size w/o insert (bp) 5169
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1100
Entrez GeneMCL1 (a.k.a. BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site blunt (destroyed during cloning)
- 3′ cloning site Sal I (not destroyed)
- 5′ sequencing primer pBabe 5'
- 3′ sequencing primer pBabe 3' (Common Sequencing Primers)
There are a few mismatches between the sequence provided and the existing NCBI sequence.
The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI.
Addgene NGS did not identify IRES sequence in plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe-Flag hMcl-1 was a gift from Roger Davis (Addgene plasmid # 25371 ; http://n2t.net/addgene:25371 ; RRID:Addgene_25371)
For your References section:Mcl-1 integrates the opposing actions of signaling pathways that mediate survival and apoptosis. Morel C, Carlson SM, White FM, Davis RJ. Mol Cell Biol. 2009 Jul . 29(14):3845-52. 10.1128/MCB.00279-09 PubMed 19433446