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pJS1
(Plasmid #253726)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253726 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    miniCTX1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dcas9
  • Species
    Streptococcus pasteurianus
  • Tag / Fusion Protein
    • 6x his (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene Plasmid #105235

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJS1 was a gift from Deborah Hung (Addgene plasmid # 253726 ; http://n2t.net/addgene:253726 ; RRID:Addgene_253726)

  • For your References section:

    An inducible CRISPRi system for phenotypic analysis of essential genes in Pseudomonas aeruginosa. Sullivan JR, Ferrara KM, Barrick R, Romano KP, Warrier T, Hung DT. mBio. 2026 Feb 11:e0276725. doi: 10.1128/mbio.02767-25. 10.1128/mbio.02767-25 PubMed 41670347
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