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pTRE3G-puro_V5-LOV-Turbo2-myc-EGFP
(Plasmid #253793)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253793 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTRE
  • Vector type
    Mammalian Expression, Lentiviral ; tetracycline inducible promoter
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    V5-LOV-Turbo2-myc-EGFP
  • Alt name
    V5-LOV-Tb
  • Alt name
    myc-EGFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2178
  • Promoter pTRE3G
  • Tag / Fusion Protein
    • V5-LOV-Turbo2-myc-EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cttcctaccctcgtaaa
  • 3′ sequencing primer catagcgtaaaaggagcaaca
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTRE3G backbone vector was gift from Alice Y. Ting (Stanford).

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2025.12.21.693523 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE3G-puro_V5-LOV-Turbo2-myc-EGFP was a gift from Xinnan Wang (Addgene plasmid # 253793 ; http://n2t.net/addgene:253793 ; RRID:Addgene_253793)

  • For your References section:

    Optogenetic Proximity Labeling Maps Spatially Resolved Mitochondrial Surface Proteomes and a Locally Regulated Ribosome Pool. Kwak CS, Du Z, Creery JS, Wilkerson EM, Major MB, Elias JE, Wang X. bioRxiv [Preprint]. 2025 Dec 23:2025.12.21.693523. doi: 10.64898/2025.12.21.693523. 10.64898/2025.12.21.693523 PubMed 41497653
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