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EGFR-vIII-ET1
(Plasmid #253822)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253822 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET1
  • Vector type
    Mammalian Expression ; pIRES2-EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFR vIII
  • Alt name
    EGFR variant III
  • Species
    H. sapiens (human)
  • Mutation
    variant III (missing most of domains I and II due to an exon 2 to 7 deletion)
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene observed 1base pair mismatch in the insert leading to K271N substitution. This discrepancy is unlikely to affect the plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFR-vIII-ET1 was a gift from Timothy Springer (Addgene plasmid # 253822 ; http://n2t.net/addgene:253822 ; RRID:Addgene_253822)

  • For your References section:

    Nanobodies targeting EGFR provide insight into conformations stabilized by glioblastoma mutations. Watson SM, Harvey EP, Pishesha N, Ploegh HL, Springer TA. J Biol Chem. 2025 Jul;301(7):110374. doi: 10.1016/j.jbc.2025.110374. 10.1016/j.jbc.2025.110374 PubMed 40516870
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