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NK812
(Plasmid #253995)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253995 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMS18
  • Backbone size w/o insert (bp) 4570
  • Total vector size (bp) 5558
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PTSG07215-5'CDS_linker_mStayGold
  • Alt name
    PTSG_07215-mStayGold
  • Species
    Salpingoeca rosetta
  • Insert Size (bp)
    988
  • Mutation
    Synonymous codon mutations made to PTSG_07215 5' CDS region (described in publication)
  • GenBank ID
    XP_004992637.1
  • Tag / Fusion Protein
    • mStayGold (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGACCATGATTACGCCAAGCTTAAGAAGAAAAGCGTAAGGCTGC
  • 3′ sequencing primer AAGACTGCAGTCGTTACGTTTCCATATGCGATGTATGTGT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NK812 was a gift from Nicole King (Addgene plasmid # 253995 ; http://n2t.net/addgene:253995 ; RRID:Addgene_253995)
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