Skip to main content
Addgene
Open Menu

pPBG01 Recombination plasmid
(Plasmid #253997)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253997 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    CloDF13
  • Backbone size w/o insert (bp) 3008
  • Total vector size (bp) 4893
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    lambdaRED
  • Species
    Lambda phage (Addgene #87359)
  • Insert Size (bp)
    1885

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer atggatattaatactgaaactgaga
  • 3′ sequencing primer tgtatttggggagcaatggcgatga
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBG01 Recombination plasmid was a gift from Julius Fredens (Addgene plasmid # 253997 ; http://n2t.net/addgene:253997 ; RRID:Addgene_253997)

  • For your References section:

    Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system. Ong S, Ghode P, Narenderan A, Lao S, Willenborg F, Eden TV, Marsh CO, Yew WS, Fredens J. Nat Microbiol. 2026 May 1. doi: 10.1038/s41564-026-02346-y. 10.1038/s41564-026-02346-y PubMed 42067623
Related items: