Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system.

Ong S, Ghode P, Narenderan A, Lao S, Willenborg F, Eden TV, Marsh CO, Yew WS, Fredens J
Nat Microbiol. 2026 May 1. doi: 10.1038/s41564-026-02346-y. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
253996	pAN29 Phagemid with EG pathway	Phagemid for LySE, carrying a metabolic pathway for ethylene glycol assimilation.
253998	pSJ55 Accessory plasmid with wt T7 DNAP	Accessory plasmid for LySE, carrying wt T7 DNAP for propagation of phage T7∆DNAP
254000	pSJ78 Phagemid with tetA	Phagemid for LySE, encoding a tetracycline efflux protein for evolution.
254001	pSJ139 Accessory plasmid with T7 DNAP v9	Accessory plasmid for LySE, carrying error-prone T7 DNAP-TadDE (v9) for optimised mutational spectrum.