Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system.

Ong S, Ghode P, Narenderan A, Lao S, Willenborg F, Eden TV, Marsh CO, Yew WS, Fredens J
Nat Microbiol. 2026 May 1. doi: 10.1038/s41564-026-02346-y. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
253996	pAN29 Phagemid with EG pathway	Phagemid for LySE, carrying a metabolic pathway for ethylene glycol assimilation.
253997	pPBG01 Recombination plasmid	Inducible lambdaRED for genomic recombination.
253998	pSJ55 Accessory plasmid with wt T7 DNAP	Accessory plasmid for LySE, carrying wt T7 DNAP for propagation of phage T7∆DNAP
254000	pSJ78 Phagemid with tetA	Phagemid for LySE, encoding a tetracycline efflux protein for evolution.
254001	pSJ139 Accessory plasmid with T7 DNAP v9	Accessory plasmid for LySE, carrying error-prone T7 DNAP-TadDE (v9) for optimised mutational spectrum.