Skip to main content
Addgene
Open Menu

pSJ78 Phagemid with tetA
(Plasmid #254000)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254000 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    p15A-phagemid
  • Backbone size w/o insert (bp) 2500
  • Total vector size (bp) 3991
  • Modifications to backbone
    Phage origin and packaging site
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    tetA
  • Species
    E. coli
  • Insert Size (bp)
    1300

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tgatcggcacgtaagaggttccaac
  • 3′ sequencing primer atggagccgggccacctcgacctga
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSJ78 Phagemid with tetA was a gift from Julius Fredens (Addgene plasmid # 254000 ; http://n2t.net/addgene:254000 ; RRID:Addgene_254000)
Related items: