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Modified pYTK075
(Plasmid #254020)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254020 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Unspecified
  • Vector type
    Synthetic Biology
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LEU2
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1917

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Modified pYTK075 was a gift from Dae-Hee Lee (Addgene plasmid # 254020 ; http://n2t.net/addgene:254020 ; RRID:Addgene_254020)

  • For your References section:

    Connector-enabled integration of Golden Gate Assembly and yeast recombination for streamlined multigene pathway construction in the biofoundry workflow. Seong MJ, Kim H, Lee H, Kim H, Lee SG, Lee MJ, Jun JS, Hong S, Kim TH, Kim SK, Shin J, Lee DH. Trends Biotechnol. 2026 Jan 14:S0167-7799(25)00502-5. doi: 10.1016/j.tibtech.2025.12.004. 10.1016/j.tibtech.2025.12.004 PubMed 41535170
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