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Tapasin-TM
(Plasmid #254045)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254045 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    psffv
  • Backbone size w/o insert (bp) 8962
  • Total vector size (bp) 10372
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tapasin-TM
  • Alt name
    TAPBP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1410
  • Mutation
    removed C terminal domain, replaced with that of HLA-G
  • Entrez Gene
    TAPBP (a.k.a. MHC1D3, NGS17, TAPA, TPN, TPSN)
  • Promoter sffv
  • Tag / Fusion Protein
    • FLAG Tag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tapasin-TM was a gift from Nikolaos Sgourakis (Addgene plasmid # 254045 ; http://n2t.net/addgene:254045 ; RRID:Addgene_254045)
  • For your References section:

    HLA-Shuttle: A system for enhancing antigen presentation in immunologically cold tumors. Hwang D, Erdman MC, Adhikari S, Pantula R, Epstein K, Li P, Costabile F, Rotsides P, Wang CS, Sun SM, Fazelinia H, Spruce LA, Fugmann T, Rrustemi T, Soh WT, Farrel A, Want MY, Facciabene A, Mir M, Maris JM, Sgourakis NG. Sci Adv. 2026 Jan 2;12(1):eaeb0821. doi: 10.1126/sciadv.aeb0821. Epub 2026 Jan 1. 10.1126/sciadv.aeb0821 PubMed 41477871