pBABE-IFNAR1-QY-tTA-SV40-puro-T2A-TagBFP
(Plasmid
#254089)
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PurposeRetroviral transfer vector for IFNAR1-tTA fusion gene expression, QY linker, puromycin selection marker, for CyCLoPs platform.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254089 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBABE-SV40-puro-T2A-TagBFP
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Vector typeRetroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIFNAR1-tTA
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SpeciesM. musculus (mouse)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE-IFNAR1-QY-tTA-SV40-puro-T2A-TagBFP was a gift from Jun Huh (Addgene plasmid # 254089 ; http://n2t.net/addgene:254089 ; RRID:Addgene_254089) -
For your References section:
In vivo detection of immune responses via cytokine activity labeling. Lu G, Zhang S, Feng M, Kim E, Cho D, Kim JH, Caris H, Silberstein L, Choi GB, Huh JR. Cell. 2026 Feb 5;189(3):939-955.e26. doi: 10.1016/j.cell.2025.12.011. Epub 2026 Jan 7. 10.1016/j.cell.2025.12.011 PubMed 41506266