MIGR1-cre-SV40-hygro
(Plasmid
#254097)
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PurposeRetroviral transfer vector for cre recombinase expression, hygromycin selection marker.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254097 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneMIGR1-SV40-hygro
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Vector typeRetroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre recombinase
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SpeciesBacteriophage P1
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MutationAA328: ATA to ATT synonymous mutation
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MIGR1-cre-SV40-hygro was a gift from Jun Huh (Addgene plasmid # 254097 ; http://n2t.net/addgene:254097 ; RRID:Addgene_254097) -
For your References section:
In vivo detection of immune responses via cytokine activity labeling. Lu G, Zhang S, Feng M, Kim E, Cho D, Kim JH, Caris H, Silberstein L, Choi GB, Huh JR. Cell. 2026 Feb 5;189(3):939-955.e26. doi: 10.1016/j.cell.2025.12.011. Epub 2026 Jan 7. 10.1016/j.cell.2025.12.011 PubMed 41506266