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pcDNA5/FRT_HaloTag7-CLIP-tag2-NLS-P2A-NLS-mTurquoise2
(Plasmid #254206)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254206 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA5/FRT
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HaloTag7-CLIP-tag2-NLS-P2A-NLS-mTurquoise2
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.03.05.709795 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT_HaloTag7-CLIP-tag2-NLS-P2A-NLS-mTurquoise2 was a gift from Kai Johnsson (Addgene plasmid # 254206 ; http://n2t.net/addgene:254206 ; RRID:Addgene_254206)

  • For your References section:

    Fast and Luminous: CLIP-tag2. Johnsson K, Nasufovic V, Pispek A, Kuehn S, Wilhelm J, Bibrowski M, Fischer J, Koch B, Kompa J, Mao R, Tarnawski M, Hiblot J. bioRxiv 2026.03.05.709795 10.64898/2026.03.05.709795
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