pPF4481
(Plasmid
#254226)
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PurposeMutagenesis plasmid encoding tnpA and the tn5 transposon harbouring AIcrVIA3
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepPF4213
- Total vector size (bp) 7384
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Modifications to backboneAcrVIA1 was removed and AIcrVIA3 inserted at the same position.
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Vector typeTransposon insertion mutagenesis vector
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAIcrVIA3
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SpeciesSynthetic
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Insert Size (bp)348
- Promoter js23100
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGCAAGGGCTGCTAAAGG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2025.12.19.695335 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPF4481 was a gift from Peter Fineran (Addgene plasmid # 254226 ; http://n2t.net/addgene:254226 ; RRID:Addgene_254226)
Map uploaded by the depositor.
